Plant material
potos-branches (Epipremnum aureum) - Branches Hemp (Cannabis sativa) medioEl sterilization medium was sterilized in the pressure cooker for 40 minutes after maximum is reached presión.Esterilización vegetalPara material sterilized plant material, follow the following instructions: -1 minutes submerged in alcohol 70% -10 minutes immersed in distilled water + bleach to 15% -3 consecutive 5-minute washes in distilled water estéril.Éste is more delicate point, because if you spend with bleach or time, the explants die, and if you do not finish you do not get any of the fungi / bacteria and your crop planting contaminan.SiembraLa explants were performed under sterile conditions by laminar flow. Planted and non-meristem explants to observe the different responses.
0 comments:
Post a Comment