Portable Skate Sharpening Machine

• LABORATORY PRACTICE INDUCTION
• 
  GLASSWARE
• 
  · Erlen Meyer : used for mixing or solutions
  · or beaker Beaker used for measuring volumes and move substances from one side to another
  · test tube, used to take samples of tissue, fungi
  · Probe: used to make measurements of volume pipette
  · : serves minimum transfer amounts
  · lighter volumes :
  solutions heats · Dropper: used to paint or to identify bacteria or fungi that is found in a plant
  · Mortar: used to marinate pettri
  · Box : used to grow mushrooms, meristems
  · funnel, is used to prevent spills
  substances · wash bottle: Substances used to wash all
  · Sieve : used to produce water droplets
  · P orta objects: used to cut objects
  · covers objects: used to cover the portal object and to see more the bottle
  . glass bottle: to install medium

matarial

· Aluminum foil, serves to cover the bottle
· vinipel Paper: used to seal the bottle
· Cotton : used to disinfect hands or objects that we use Indicator Tape
· : used to indicate the temperature in the sterilization
· Newsprint : used to wrap any object which bottle or to sterilize us to go
· Gasa : used to make plugs and sterilized


ACCESSORIES
· Spatula: Used to transport, mix and remove solid solutions
· Knife: used to cut the corm
· visturi : used to cut tiny particles meristem tip
· handle : used for fungi and bacteria pass from one side to another
· Brush: used for cleaning utensils

SUPPLIES (consumable)
· 70% Alcohol: Used to disinfect
· 90% Alcohol: Used to fuel
· Agar : is a seaweed extract rich in nutrients, used to prepare the culture medium
· Glucose 28gr/litro : powdered sugar, is a substance produced by plants THROUGH THE trtansformacion of light energy into chemical energy brings nutrients to the
medium-Plant hormones: auxins present in higher consentracion in the growing points of plants or in meristematic tissue.

· gibberellic acid: used to induce rooting and to promote seed germination.

-Macronutrients: major and minor elements

EQUIPMENT

to be considered a team needs to have power or power to do work.

- heating plate: culture media to heat or substances
· Auto key: used to sterilize utensils and accessories, is sterilized at 120C °. This is pressure gauge valve. Value key self $ 3,000,000.
· electronic balance. used to measure exact amount of weight
· blender :
· Peachimetro : to measure the pH of the solutions (hydrogenation potential PH)
· Refrigerator: used to preserve samples.

- micropipette: to measure microliter
· Microscope: used to see things not visible to the naked eye.
· Macroscope or stereoscope : Allows you to see things gross.
· laminar flow chamber, used to disinfect

SAMPLING PROCESS

The sampling process is performed in three area of \u200b\u200bthe laboratory are:

presampling Area Area sampling



laminar chamber
3. Area

Micro meristem propagation characteristics ensures good for the farmer are
1. A massive increase in plant quality
2. plant health guarantee
3. ensures good agronomic characteristics
4. Ciency guarantees a good quality


Some characteristics of monocotiledonia
· herbaceous stem leaves
· peninervada
·
fasciculate root feature of dicot woody stem
·
· sheet peninervada
·
taproot
THE presampling
has three stages are
1. Initiation
2. Differentiations
3. Multiplication
4. Climate

STEPS TO GET A MERISTEMS

1. plant is selected
nice features

Already
2. laboratory is due disinfection and corm selection



3. cut was taken in equal proportions on all four sides ensuring not to damage the growing point.



4. As you cut is disinfected in solutions previously prepared



5. Already having the meristem, it is placed in the culture medium.

The meristem remains in the culture medium for about two months and grows according to the nutrients that have been dispusto, from the moment the plant starts to present its new growth which makes them a microdesmache; and placed in other media, eSATA is a multiplication plant.

To ensure a good plant should get up to four new plants, there are mutations can occur later.

PRACTICE WAS IN CHARGE OF THE BARRIOS OBREGON INGENIRA MONICA WHO IS IN CHARGE OF Labortories.